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KMID : 0355519860100020089
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Korean Journal of Oral Biology
1986 Volume.10 No. 2 p.89 ~ p.98
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EFFECTS OF LIDOCAINE AND DIAZEPAM ON THE CONTENTS OF 5-HYDROXYTRYPTAMINE AND 5-HYDROXYINDOLE 3-ACETIC ACID IN SEVERAL REGIONS OF THE RAT BRAIN
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Abstract
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We tried to clarify the role of serotonergic neurons in lidocaineinduced convulsion and in the anticonvulsant action of diazepam. Fifty SpragueDawley rats were grouped in 4 groups and two ´injections were made in 10 minuteinterval to each group. Saline and saline(saline control), saline and lidocaine 70mg/kg (lidocaine group), diazepam 5mg/kg and saline (diazepam group) or diazepam 5mg/kg and lidocaine 70mg/kg (dz+lido group) were administered intraperitoneally and the rats were Eacrificed at 3, 6 or 12 minute after second injection. 5HT and 5HIAA concentration in the brain tissue was measured by HPLCEC detection method and the 5HIAA/5 HT ratio was calculated. The results were as follows
1. The concentrations of 5HT and 5HIAA were significantly increased in hippocampus and in medulla and pons by the administration of lidocaine and the 5HIAA/5HT ratio was increased in hippocampus and in amygdala.
2. By the pretreatment with diazepam the increase in 5HIAA/5-HT ratio by lidocaine was reversed in all regions examined and the concentrations of the 5HT and 5HIAA were increased when compared with saline control.
3. Diazepam increased the concentrations of 5HT and 5HIAA in all regions examined and the increase was significant in medulla and pons at 6 minutes and in amygdala and hippocampus at 12 minute. The 5 HIAA/5 HT ratio was somewhat higher than dz+lido group and saline control group but somewhat lower than lidocaine group.
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KEYWORD
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